Bioluminescence Imaging System for Circadian Rhythmicity

Major Functions / Key Features

Nikon Inverted Microscope Ti2-E Imaging System coupled with LumiCycle System 

• Motorized and intelligent model for advanced imaging applications. Compatible with PFS, auto correction collar, and external phase contrast system. The base of choice for live-cell imaging, high-content applications, confocal and super-resolution.
• LumiCycle performs high-throughput luminometry on self-luminous tissues, such as those from transgenic animals containing the luciferase gene.
• The system is equipped with 4 photon-counting photomultiplier tubes (PMTs), each selected for low dark counts and high sensitivity in the green portion of the spectrum at which luciferase emits light. 32 tissue samples, each in a 35 mm Petri dish, can be counted at one time. 8 samples share each photodetector using an automated turntable that alternately brings each sample under the detector one at a time. The apparatus fits inside a standard incubator. An internal fan circulates the incubator air to maintain the proper temperature within the chamber. 
• The turntable and photon counting are fully automated. System setup and operation is straightforward. In addition to its high precision photon counting hardware, LumiCycle has the most flexible and easy-to-use software for the collection and analysis of circadian rhythms in luminometry data.