Nikon Eclipse Ti2-E Live-cell Fluorescence Imaging System

Specifications

Major Functions / Key Features Location Fee Other Information

Major Functions / Key Features

The Nikon Eclipse Ti2-E live-cell imaging system is an inverted, fully motorised, large field-of-view wide-field fluorescence microscope equipped with a collection of state-of-the-art features for live-cell and large-tissue tile imaging.

Specifications:

Large (i.e., 25 mm) field-of-view (FOV)
Fly-eye lens design to provide uniform illumination across the whole FOV
High-precision motorised stage with encoder
Adaptive auto-focusing system (Nikon Perfect Focus System)
Real-time deconvolution and deblurring
Multi-well plate holder and high-content imaging software module for high-throughput imaging and data analysis
Tile scanning and image stitching to produce high-resolution overview image of large tissue sections
Environmental chamber with temperature and CO₂ concentration control (Tokai Hit STX series)
Motorised condenser, objective and fluorescence filter turrets

Location

Y708a

Fee

Internal users: $58
Collaborators: $116
External users: $290
Discount for long-duration bookings:
Applicable to consecutive bookings of 8 hours or longer
A 50% discount will be applied to any segments that fall within non-office hours
- (i.e., from 6 pm to 9 am on weekdays, and the whole days on Saturdays, Sundays and public holidays)
Price adjustments, if any, will be made after the completion of the imaging session
The ULS reserves the right to interpret these terms
Consumables:
Marienfeld no. 1.5H 18 mm round coverglass ($148; 100 pcs)
MatTek 35 mm no. 1.5 glass bottom culture dish ($190; 10 pcs)
Nunc Lab-Tek II no. 1.5 8-well chambered coverglass ($435; 8 pcs)
ProLong Diamond antifade mountant ($372; 2 mL)
ProLong Live antifade reagent ($333; 1 mL)
EMS 16% paraformaldehyde ($116; 10 mL)