Introduction:
Bruker timsTOF Pro 2 Mass Spectrometer is a high resolution mass spectrometer that has two distinct separation and sequencing technologies - Trapped Ion Mobility Spectrometry (TIMS) and Parallel Accumulation Serial Fragmentation (PASEF). TIMS add additional dimension of spearation on top of LC-MS to resolve sample complexity. Ions are accumulated and released based on their mobility (collisional cross section (CCS) of ions). With PASEF technology >100 Hz sequencing speed can be achieved with near 100% duty cycle which is nearly no ions loss so as to enhance sensitivty, peak capacity and characterize compounds in high confidence.
Specification:
Dionex UltiMate 3000 RSLCnano
- Binary nano pump with continuous direct nano flow
- Flow rate range: 20 nL/min – 50 µL/min
- Column compartment: 10C – 75C
Dionex UltiMate 3000 RSLCnano
- Binary pump with continuous flow
- Flow rate range: 0.001 mL/min – 8 mL/min
- Column compartment: 10C – 75C
Bruker timsTOF Pro 2 Mass Spectrometer
- Ion Source:
- Electrospray ionization (ESI)
- nanoelectrospray ionization (CaptiveSpray, CaptiveSpray nanoBooster)
- Fragementation type:
- Collision induced dissociation (CID)
- Mass Analyzer:
- Quadrupole Time-of-flight (QTOF)
- Scan modes
- Data dependent acquisition PASEF (dda-PASEF)
- Data independent acquisition PASEF (dia-PASEF)
- Parallel Reaction Monitoring PASEF (prm-PASEF)
- Multiple Reaction Monitoring (MRM)
- Broad-band collision-induced dissoviation (bbCID)
- Mass range:
- m/z 20-40,000
- Resolution:
- 60,000 FSR (full sensitivity resolution) at m/z 1,200
- Mass accuracy:
- Less than 2 ppm RMS using external calibration
- Less than 0.8 ppm RMS using internal calibration
- TIMS accuracy:
- Less than 0.5% using internal calibration
- Less than 1% RSD using external calibration
- Sensitivity:
- Signal-to-noise ratio more than 100:1 using 2.5 fmol of Glu-Fibrinopeptide B
- Acquisition rate:
- More than 120 Hz in PASEF mode
Application:
- Shotgun Proteomics
- Glycoproteomics
- Phosphoproteomics
- Lipidomics
- Isomer Separation
Software:
- FragPipe with MSFragger (for Proteomics, dda-PASEF)
- MaxQuant (for dda-PASEF)
- Spectronaut 15 (for DIA Proteomics)
- DIA-NN (for DIA Proteomics)
- Skyline (dia-PASEF)
Note to user:
First-time users must be trained by equipment-in-charge before they can use the instrument independently.
Samples should be desalted and purified prior to MS analysis to enhance the detection sensitivity and minimize contamination.
Booking:
Please visit our University Research Facility Management System (URFMS) website for registration, training request and booking arrangement.
Contact information:
Location: Y1309, Block Y, The Hong Kong Polytechnic University
Equipment-in-charge: Dr. Sirius TSE (Office: U514)/ Dr. Chi-Hang CHOW (Office: U003)
Email: sirius.tse@polyu.edu.hk/ chihang.chow@polyu.edu.hk
Telephone: 3400 8878/ 34008813
