Leica TCS SPE Confocal Microscope

Confocal microscopy is a powerful tool for generating high resolution images and 3-D reconstruction of a specimen. It offers several advantages over conventional widefield optical microscopy, including the ability to control depth of field by adjusting the pinhole size, elimination or reduction of background information away from the focal plane, and the capability to collect serial optical sections from thick specimens.

Specifications:

Manual inverted microscope (Model: Leica DMi8) with TCS SPE confocal module capable of imaging with prism-based spectral detection
Objectives: 10×/0.3 dry; 40×/1.15 oil; 63×/1.3 oil
4 laser lines available: 405 nm, 488 nm, 561 nm, 635 nm (solid state laser)
Internal detector: 1 PMT detector
Mercury metal-halide bulb for fluorescence viewing through the eyepieces; filter cubes: DAPI, FITC, Rhod
LAS AF software
Motorized focus drive

Hourly rate

Internal users: $142
Collaborators: $284
External users: $710

Location

Y708

Staff-in-charge

Dr. Michael Yuen