Agilent Seahorse XFe24 Extracellular Flux Analyser

The Seahorse XFe24 extracellular flux analyser measures oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), at intervals of approximately 5-8 minutes, of live cells in a 24-well plate format. OCR is a key indicator of mitochondrial respiration and ECAR reflects glycolysis. It is an important instrument that provides a system-level view of cellular metabolic function in cultured living cells, islets, and ex vivo samples.

A sensor cartridge setup for Seahorse Analyser

Specifications:

Real-time results of OCR and ECAR
Live-cell response in a specialised 24-well microplate
4 injection ports
Ability to analyse as few as 10,000 cells per well

Hourly rate

Internal users: $76
Collaborators: $152
External users: $380

Location

Y717b

Recent publications

Year	Title	Journal	Authors
2023	Perturbation of Autophagy by a Beclin 1-Targeting Stapled Peptide Induces Mitochondria Stress and Inhibits Proliferation of Pancreatic Cancer Cells	Cancers	Li, N., et al.
2022	RASA2 ablation in T cells boosts antigen sensitivity and long-term function	Nature	Julia, C., et al.
2022	Carbon source availability drives nutrient utilization in CD8+ T cells	Cell Metabolism	Irem, K., et al.
2022	Abelson tyrosine kinase controls BCR signalling and B-cell differentiation by promoting B-cell metabolism	Immunology	Luo, L., et al.

Staff-in-charge

Z215
34008057
hoyin.chow@polyu.edu.hk