iBright™ FL1500 Imaging System

Name of the equipment

iBright™ FL1500 Imaging System

Brand

Invitrogen™

Description

Invitrogen™ iBright™ FL1500 Imaging System has powerful features that help make imaging and analyzing western blots and gels easy. The high-resolution, 9.1-megapixel (MP) camera and suite of automated features can help you produce publication-quality data fast. The touchscreen interface is crafted to provide a smooth image capture experience. The onboard software and standalone Invitrogen™ iBright™ Analysis Software are designed to streamline image analysis.

Specification

Smart Exposure technology rapidly determines the optimal exposure time, and helps minimize the risk of overexposure or underexposure, by maximizing pixel intensity while avoiding saturation. It is available with all modes in all five detection modes. The optimal exposure time for each channel is determined separately in Fluorescent Blots and Universal modes. It can also be applied to a specific region of a gel or blot.

Smart Range™ HDR (high dynamic range) technology can help maximize the linear dynamic range of chemiluminescent western blot images when sample proteins have widely varying expression levels.

Five fluorescence channels allow multiplexing with up to four fluorophores that emit in the visible and NIR ranges.

Automatic sample rotation, autozoom and autofocus functions are available for easing imaging.

Multiple channels for colorimetric signals, fluorescence and chemiluminescence are available for imaging signals from gel and membrane.

It enables automated and customizable quantitation and normalization to monitor or mathematically compensate for experimental or sample variability. This includes normalization based on housekeeping proteins (HKPs) and total lane protein.

Standard iBright Analysis Software is freely available in a desktop version that is compatible with Windows™ and macOS™ operating systems.

Applications

The iBright Imaging System has up to five imaging modes to support multiple applications. A visible image of the gel or membrane is automatically captured in each imaging mode, which can simplify image analysis workflows like molecular weight analysis. The Imaging mode includes the following,

1. Protein Gels Colorimetric signals from stains on gels and membranes; fluorescence signals from stains
2. Nucleic Acid Gels Signals from a variety of fluorescent nucleic acid stains
3. Chemi Blots Chemiluminescence from horseradish peroxide (HRP) and alkaline phosphatase (AP) substrates
4. Fluorescence Blots Fluorescence from visible and near-infrared fluorophores
5. Universal Custom mode to image with multiple stains (chemiluminescence, fluorescence, colorimetric signals, and/or visible signals)

Contact information

PIC: Dr. Stanley CHEUNG

Email: kwok-kuen-stanley.cheung@polyu.edu.hk

Office: TU305