Nikon Ti2-E Live-cell Imaging System

The Nikon Eclipse Ti2-E live-cell imaging system is an inverted, fully motorised, large field-of-view wide-field fluorescence microscope equipped with a collection of state-of-the-art features for live-cell and large-tissue tile imaging.

Nikon Eclipse Ti2-E Live-cell Fluorescence Imaging System

Specifications:

Large (i.e., 25 mm) field-of-view (FOV)
Fly-eye lens design to provide uniform illumination across the whole FOV
High-precision motorised stage with encoder
Adaptive auto-focusing system (Nikon Perfect Focus System)
Real-time deconvolution and deblurring
Multi-well plate holder and high-content imaging software module for high-throughput imaging and data analysis
Tile scanning and image stitching to produce high-resolution overview image of large tissue sections
Environmental chamber with temperature and CO₂ concentration control (Tokai Hit STX series)
Motorised condenser, objective and fluorescence filter turrets
Imaging and contrasting modes:

Bright-field
Phase contrast
Differential interference contrast (DIC)
Wide-field fluorescence

Multi-wavelength LED illumination system (CoolLED pE-4000) offering 16 selectable wavelengths across 4 channels:

Channel 1: 365/385/405/435 nm
Channel 2: 460/470/490/500 nm
Channel 3: 525/550/580/595 nm
Channel 4: 635/660/740/770 nm

Fluorescence filter sets:

Objectives (details):

CFI Plan Fluor 4×/0.13 Dry
CFI Plan Fluor 10×/0.30 Dry
CFI Super Plan Fluor 20×/0.45 Dry
CFI Super Plan Fluor 20×/0.70 Dry (on request)
CFI Super Plan Fluor 40×/0.60 Dry
CFI Plan Apo 20×/0.75 Dry
CFI Plan Apo 40×/0.95 Dry (on request)
CFI Plan Apo 60×/1.40 Oil

Nikon DS-Qi2 CMOS camera:

Large-format (36 × 23.9 mm) and optimised for 25-mm FOV
High pixel density (2424 × 2424 pixels)
High quantum efficiency (QE; ~77% at peak)
High-speed (6 fps at full resolution or 45 fps with 3×3 pixel averaging)

Teledyne Photometrics Kinetix back-illuminated scientific CMOS (sCMOS) camera:

Large-format (20.8 × 20.8 mm) and optimised for 25-mm FOV
High pixel density (3200 × 3200 pixels)
Extremely high QE (~96% at peak)
Extremely high-speed (498 fps at full resolution)

Usage rate

Internal users: $58
Collaborators: $116
External users: $290

Discount for long-duration bookings:

Applicable to consecutive bookings of 8 hours or longer
A 50% discount will be applied to any segments that fall within non-office hours (i.e., from 6 pm to 9 am on weekdays, and the whole days on Saturdays, Sundays and public holidays)
Price adjustments, if any, will be made after the completion of the imaging session
The ULS reserves the right to interpret these terms

Consumables:

Marienfeld no. 1.5H 18 mm round coverglass ($148; 100 pcs)
MatTek 35 mm no. 1.5 glass bottom culture dish ($190; 10 pcs)
Nunc Lab-Tek II no. 1.5 8-well chambered coverglass ($435; 8 pcs)
ProLong Diamond antifade mountant ($372; 2 mL)
ProLong Live antifade reagent ($333; 1 mL)
EMS 16% paraformaldehyde ($116; 10 mL)

Location

Y708a

Staff-in-charge

Dr. Michael Yuen

Senior Scientific Officer

Dr. Idy Ho

Scientific Officer

Z215
34008985
idyht.ho@polyu.edu.hk