Circular Dichroism (CD) spectroscopy is one of the most important techniques for the characterization of proteins’ secondary structure, and the determination of absolute configuration and stereochemical analysis of biomolecules, organic molecules and inorganic complexes. CD is an absorption spectroscopic method that utilizes circularly polarized light to study the structural aspects of a chiral molecule (i.e. the structure of a molecules and its mirror image is not superimposable to each other). Circularly polarized light consists of both left- and right-handed direction. The chiral chromophores on a molecule preferentially absorb one of the direction of the light. Therefore, based on the differential absorption of left and right circularly polarized light, the stereochemical information of the molecule can be extracted.