Nikon Ti2-E Live-cell Imaging System

Introduction:

The Nikon Eclipse Ti2-E live-cell imaging system is a fully motorized, inverted fluorescence microscope with a large field-of-view and state-of-the-art features that make it ideal for live-cell and large-tissue tile imaging. Equipped with advanced technology, this system is designed to meet the needs of cutting-edge research in various scientific fields.

With its large field-of-view and high-resolution imaging capabilities, the Nikon Eclipse Ti2-E live-cell imaging system provides outstanding image quality for dynamic imaging studies and wide-area imaging tasks. This system has been designed with an array of advanced features, such as motorized focus and sample-positioning stages, which not only simplify imaging workflows but also improve imaging acquisition speed and accuracy.

Moreover, the Nikon Eclipse Ti2-E live-cell imaging system has been optimized for live-cell imaging studies in diverse applications, including cell biology, tissue engineering, developmental biology, neuroscience, and drug discovery, among others. Its versatility enables researchers to perform time-lapse imaging of cellular events, track cell movement, and study intracellular dynamics with pinpoint accuracy.

The Nikon Eclipse Ti2-E Live-cell Fluorescence Imaging System is a cutting-edge microscope that features state-of-the-art components for high-resolution, large field-of-view imaging. Its fly-eye lens design ensures uniform illumination across the entire 25 mm field-of-view, while its high-precision motorized stage with encoder adaptive auto-focusing system (Nikon Perfect Focus System) provides accurate positioning and sharp, in-focus images.

The system also includes tile scanning and image stitching capabilities to produce high-resolution overview images of large tissue sections, as well as an environmental chamber with precise temperature and CO2 concentration control (Tokai Hit STX series) for live-cell imaging studies. Additionally, the motorised condenser objective and fluorescence filter turrets allow for a variety of imaging and contrasting modes, including bright-field, differential interference contrast (DIC), and wide-field fluorescence.

At our laboratory, we have utilized the Nikon Eclipse Ti2-E Live-cell Fluorescence Imaging System to conduct advanced research projects in various scientific fields, including cell biology, neuroscience, and drug discovery. We believe that the system's advanced features and imaging capabilities make it an indispensable tool for researchers who require high-quality, large field-of-view imaging.

Specifications:

• The microscope system supports brightfield, DIC, and fluorescence modes of microscopic observation.
• It utilizes an advanced fully motorized research-grade inverted microscope with a Z-axis focusing mechanism, intelligent 1.5x variable magnification lens, and intelligent Bosch lens. The microscope features status indicator lights and control buttons for the motorized components. It has networking capabilities for real-time control through smart devices such as tablets.
• The microscope's optical system adopts the advanced CFI60 independent chromatic aberration correction infinity optical system, with each optical component being independent and automatically corrected for chromatic aberration. This design ensures high-quality images and offers maximum flexibility and expandability.
• It has a motorized 4-position light path switch with 100% eyepiece, 100% left port, 100% right port (both sides with a 25mm imaging field of view), and 20% eyepiece/80% left port beam splitting. The microscope also supports various expansion interfaces, including rear ports.
• The Z-axis travel distance is 10mm, and the Z-axis adjustment step is a minimum of 10nm.
• The microscope features a millisecond-level active focus stabilization system that tracks the focal plane in real-time. It is compatible with all observation modes and objective lenses. It uses an 855nm infrared correction laser for wide adaptability to fluorescent dyes and is compatible with glass and plastic bottom culture dishes.
• It has an expandable multi-layer optical path design, providing unparalleled expansion configuration capabilities.
• The eyepiece tube is ergonomically designed and allows for adjustable interpupillary distance. The field of view is 22mm.
• The transmitted light source is a high-power, long-life LED illumination with built-in compound eyepiece lenses to ensure uniform illumination of a large field of view.
• It has a seven-hole motorized condenser turret.
• The motorized DIC attachment can detect the status of the polarizer.
• It features a fully automatic high-precision encoded stage, with an effective travel range of Y ±57mm, and X ±36.5mm. The maximum speed is 25mm/s.
• The motorized stage can be controlled through confocal software, with an automatic scanning controlTi2-E Inverted Microscope Parameters:
• The microscope is equipped with a six-position motorized objective changer that allows for automatic parfocal adjustment between objectives. It also has a built-in intelligent detection DIC slide slot.
• Four objective lenses meet various high-end applications (all objective lenses can be used with the focus stabilization system)
  • 10X Plan Apochromatic Objective Lens, N.A. ≥ 0.45
  • 20X Plan Apochromatic Objective Lens, N.A. ≥ 0.75, with Z-axis UV correction capability
  • 40X Plan Apochromatic Objective Lens, N.A. ≥ 1.15, water immersion
  • 60X Plan Apochromatic Objective Lens, N.A. ≥ 1.40, oil immersion, with Z-axis UV correction capability
• Six-position motorized fluorescence filter turret, with built-in motorized shutter.
• Research-grade "L-type" right-side lamp house design for fluorescence, equipped with aperture and field diaphragm adjustments; built-in high-transmission compound eye illumination lens ensures uniform illumination of a large fluorescence field of view.
• Fluorescence light source: 130W mercury lamp, can be continuously used (with a lifespan) of over 2000 hours through fiber optic connection.
• Fluorescence filter set, easy to replace, covers UV and visible light wavelengths.
  • DAPI UV excitation filter
  • FITC blue excitation filter
  • TRITC green excitation filter
  • CY5 far-red excitation filter